This article summarizes all aspects concerning production of the rhizobium biofertilizer. The use of biofertilizer is low cost when compared to chemical fertilizer pseudomonas fluorescens are the most important components of biofertilizer. Strategies for mass production of inocula 485 immobilization of spores and hyphae. Mass production of bacterial biofertilizer the mass production of carrier based bacterial biofertilizers involves three stages. Rhizobium inoculants of legumes will enhance yields in. Mother cultures carrier material lignite or bentonite or peat of desired quality in powder form 70100 mesh pet bottles of desired quantity, cardboard cortans growth materials include manital, sucrose and chemical nutrients. This article summarizes all aspects concerning production of the rhizobium biofertilizer okadin taking place at the environment, natural resources and desertification research institute endri. Pdf rhizobium biofertilizer okadin production and future.
Under optimum conditions this population level could be attained with in 4 to 5 days for rhizobium. Rhizobium, biofertilizer production, symbiotic nitrogen fixing bacteria, carrier material. Mass production and quality control of microbial inoculants. Rhizobium bio fertilizer mass production by pavan kundur p c. The production of rhizobium, biofertilizer in the treatment to. Culturing of microorganisms processing of carrier material mixing the carrier and the broth culture and packing culturing of microorganisms rhizobium. Molasses growth medium for production of rhizobium sp. In order to meet the food requirements of ever increasing population, the nitrogen fertilizer requirement for crop production by 2000 a. Here, research helps identify the most effective strain of rhizobium that is then cultured for large scale production. Mass cultivation of microbial inoculants biofertilizers. Biofertilizer mass cultivation of microbial inoculants bacteria to be inoculated in soil as biofertilizer need to be multiplied on artificial media to harvest on a large scale so that it can be supplied to farmers. Pdf this article summarizes all aspects concerning production of the rhizobium biofertilizer okadin. The culture is incubated until maximum cell population of 1010 to 1011 cfuml is produced.
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